pSicoR PGK Puro慢病毒载体使用说明

编号 北京华越洋VECT231323 载体名称 pSicoR PGK Puro

pSicoR PGK Puro载体基本信息：

pSicoR PGK puro, pSicoR-PGK-puro 载体名称:

质粒类型: 哺乳动物细胞慢病毒表达载体，RNAi, Cre/loxP 高拷贝/低拷贝: 高拷贝

CMV 启动子:

克隆方法: 多克隆位点，性内切酶

7696 bp 载体大小:

5' 测序引物及序列: mU6-F：CAGCACAAAAGGAAACTCACC

-- 3' 测序引物及序列:

-- 载体标签:

载体抗性: 氨苄 筛选标记: 嘌呤霉素

本载体利用表达的嘌呤霉素作为筛选标记。嘌呤霉素和shRNA寡核苷酸被LoxP位点加在中间。Cre酶

备注:

能够同时使得这两个基因在重组过程中切除出载体，同时启动shRNA的表达。 / 稳定性:

-- 组成型:

病毒/非病毒: 慢病毒

pSicoR PGK Puro载体质粒图谱和多克隆位点信息 pSicoR PGK Puro载体简介：

pSicoR PGK puro载体允许条件控制（通过Cre-LoxP）, 稳定表达shRNAs，能够RNA干扰细胞和转基因小鼠的基因表达。Cre序列的加入能够使shRNA关闭表达。shRNA编码寡核苷序列可以通过HpaI和XhoI性内切酶位点插入载体。

pSicoR PGK Puro载体序列：

ORIGIN -

1 GCTTAAGCGG TCGACGGATC GGGAGATCTC CCGATCCCCT ATGGTGCACT CTCAGTACAA 61 TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATCTGCTCCC TGCTTGTGTG TTGGAGGTCG 121 CTGAGTAGTG CGCGAGCAAA ATTTAAGCTA CAACAAGGCA AGGCTTGACC GACAATTGCA 181 TGAAGAATCT GCTTAGGGTT AGGCGTTTTG CGCTGCTTCG CGATGTACGG GCCAGATATA 241 CGCGTTGACA TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC 301 ATAGCCCATA TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC 361 CGCCCAACGA CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA 421 TAGGGACTTT CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG

481 TACATCAAGT GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC 541 CCGCCTGGCA TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT 601 ACGTATTAGT CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG 661 GATAGCGGTT TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT 721 TGTTTTGGCA CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA 781 CGCAAATGGG CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT 841 ACTGGGTCTC TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC 901 CCACTGCTTA AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG 961 TTGTGTGACT CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT 1021 AGCAGTGGCG CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC 1081 GCAGGACTCG GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA 1141 CGCCAAAAAT TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA 1201 TTAAGCGGGG GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA 1261 AAAAATATAA ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA 1321 ATCCTGGCCT GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT 1381 CCCTTCAGAC AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT 1441 GTGTGCATCA AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG 1501 AGCAAAACAA AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCGCTGAT CTTCAGACCT 1561 GGAGGAGGAG ATATGAGGGA CAATTGGAGA AGTGAATTAT ATAAATATAA AGTAGTAAAA 1621 ATTGAACCAT TAGGAGTAGC ACCCACCAAG GCAAAGAGAA GAGTGGTGCA GAGAGAAAAA 1681 AGAGCAGTGG GAATAGGAGC TTTGTTCCTT GGGTTCTTGG GAGCAGCAGG AAGCACTATG 1741 GGCGCAGCGT CAATGACGCT GACGGTACAG GCCAGACAAT TATTGTCTGG TATAGTGCAG 1801 CAGCAGAACA ATTTGCTGAG GGCTATTGAG GCGCAACAGC ATCTGTTGCA ACTCACAGTC 1861 TGGGGCATCA AGCAGCTCCA GGCAAGAATC CTGGCTGTGG AAAGATACCT AAAGGATCAA 1921 CAGCTCCTGG GGATTTGGGG TTGCTCTGGA AAACTCATTT GCACCACTGC TGTGCCTTGG 1981 AATGCTAGTT GGAGTAATAA ATCTCTGGAA CAGATTTGGA ATCACACGAC CTGGATGGAG 2041 TGGGACAGAG AAATTAACAA TTACACAAGC TTAATACACT CCTTAATTGA AGAATCGCAA 2101 AACCAGCAAG AAAAGAATGA ACAAGAATTA TTGGAATTAG ATAAATGGGC AAGTTTGTGG 2161 AATTGGTTTA ACATAACAAA TTGGCTGTGG TATATAAAAT TATTCATAAT GATAGTAGGA 2221 GGCTTGGTAG GTTTAAGAAT AGTTTTTGCT GTACTTTCTA TAGTGAATAG AGTTAGGCAG 2281 GGATATTCAC CATTATCGTT TCAGACCCAC CTCCCAACCC CGAGGGGACC CGACAGGCCC 2341 GAAGGAATAG AAGAAGAAGG TGGAGAGAGA GACAGAGACA GATCCATTCG ATTAGTGAAC 2401 GGATCGGCAC TGCGTGCGCC AATTCTGCAG ACAAATGGCA GTATTCATCC ACAATTTTAA 2461 AAGAAAAGGG GGGATTGGGG GGTACAGTGC AGGGGAAAGA ATAGTAGACA TAATAGCAAC 2521 AGACATACAA ACTAAAGAAT TACAAAAACA AATTACAAAA ATTCAAAATT TTCGGGTTTA 2581 TTACAGGGAC AGCAGAGATC CAGTTTGGTT AGTACCGGGC CCGCTCTAGA GATCCGACGC 21 CGCCATCTCT AGGCCCGCGC CGGCCCCCTC GCACAGACTT GTGGGAGAAG CTCGGCTACT 2701 CCCCTGCCCC GGTTAATTTG CATATAATAT TTCCTAGTAA CTATAGAGGC TTAATGTGCG 2761 ATAAAAGACA GATAATCTGT TCTTTTTAAT ACTAGCTACA TTTTACATGA TAGGCTTGGA 2821 TTTCTATAAC TTCGTATAGC ATACATTATA CGAAGTTATA CATGTCACAA AAGGAAACTC 2881 ACCCTAACTG TAAAGTAATT GTGTGTTTTG AGACTATAAA TATCCCTTGG AGAAAAGCCT 2941 TGTTAACGCG CGGTGACCCT CGAGTACTAG GATCCATTAG GGAATTCGTC GACCTCGAAA 3001 TTCTACCGGG TAGGGGAGGC GCTTTTCCCA AGGCAGTCTG GAGCATGCGC TTTAGCAGCC 3061 CCGCTGGGCA CTTGGCGCTA CACAAGTGGC CTCTGGCCTC GCACACATTC CACATCCACC

3121 GGTAGGCGCC AACCGGCTCC GTTCTTTGGT GGCCCCTTCG CGCCACCTTC TACTCCTCCC 3181 CTAGTCAGGA AGTTCCCCCC CGCCCCGCAG CTCGCGTCGT GCAGGACGTG ACAAATGGAA 3241 GTAGCACGTC TCACTAGTCT CGTGCAGATG GACAGCACCG CTGAGCAATG GAAGCGGGTA 3301 GGCCTTTGGG GCAGCGGCCA ATAGCAGCTT TGCTCCTTCG CTTTCTGGGC TCAGAGGCTG 3361 GGAAGGGGTG GGTCCGGGGG CGGGCTCAGG GGCGGGCTCA GGGGCGGGGC GGGCGCCCGA 3421 AGGTCCTCCG GAGGCCCGGC ATTCTGCACG CTTCAAAAGC GCACGTCTGC CGCGCTGTTC 3481 TCCTCTTCCT CATCTCCGGG CCTTTCGACC TGCATCCATC TAGATCTCGA TCGAGCAGCT 3541 GAAGCTTACC ATGACCGAGT ACAAGCCCAC GGTGCGCCTC GCCACCCGCG ACGACGTCCC 3601 CAGGGCCGTA CGCACCCTCG CCGCCGCGTT CGCCGACTAC CCCGCCACGC GCCACACCGT 3661 CGATCCGGAC CGCCACATCG AGCGGGTCAC CGAGCTGCAA GAACTCTTCC TCACGCGCGT 3721 CGGGCTCGAC ATCGGCAAGG TGTGGGTCGC GGACGACGGC GCCGCGGTGG CGGTCTGGAC 3781 CACGCCGGAG AGCGTCGAAG CGGGGGCGGT GTTCGCCGAG ATCGGCCCGC GCATGGCCGA 3841 GTTGAGCGGT TCCCGGCTGG CCGCGCAGCA ACAGATGGAA GGCCTCCTGG CGCCGCACCG 3901 GCCCAAGGAG CCCGCGTGGT TCCTGGCCAC CGTCGGCGTC TCGCCCGACC ACCAGGGCAA 3961 GGGTCTGGGC AGCGCCGTCG TGCTCCCCGG AGTGGAGGCG GCCGAGCGCG CCGGGGTGCC 4021 CGCCTTCCTG GAGACCTCCG CGCCCCGCAA CCTCCCCTTC TACGAGCGGC TCGGCTTCAC 4081 CGTCACCGCC GACGTCGAGG TGCCCGAAGG ACCGCGCACC TGGTGCATGA CCCGCAAGCC 4141 CGGTGCCTGA CGCCCGCCCC ACGACCCGCA GCGCCCGACC GAAAGGAGCG CACGACCCCA 4201 TGCATCGATG ATATCATAAT TTAAACAAGC AAAACCAAAT TAAGGGCCAG CTCATTCCTC 4261 CCACTCATGA TCTATAGATC TATAGATCTC TCGTGGGATC ATTGTTTTTC TCTTGATTCC 4321 CACTTTGTGG TTCTAAGTAC TGTGGTTTCC AAATGTGTCA GTTTCATAGC CTGAAGAACG 4381 AGATCAGCAG CCTCTGTTCC ACATACACTT CATTCTCAGT ATTGTTTTGC CAAGTTCTAA 4441 TTCCATCAGA AGCTGGTCGA CTCTAGCTAG ATGCGCGGCC GCGTCGAGGG ACCTAATAAC 4501 TTCGTATAGC ATACATTATA CGAAGTTATA CATGTTTAAG GGTTCCGGTT CCACTAGGTA 4561 CAATTCGATA TCAAGCTTAT CGATAATCAA CCTCTGGATT ACAAAATTTG TGAAAGATTG 4621 ACTGGTATTC TTAACTATGT TGCTCCTTTT ACGCTATGTG GATACGCTGC TTTAATGCCT 4681 TTGTATCATG CTATTGCTTC CCGTATGGCT TTCATTTTCT CCTCCTTGTA TAAATCCTGG 4741 TTGCTGTCTC TTTATGAGGA GTTGTGGCCC GTTGTCAGGC AACGTGGCGT GGTGTGCACT 4801 GTGTTTGCTG ACGCAACCCC CACTGGTTGG GGCATTGCCA CCACCTGTCA GCTCCTTTCC 4861 GGGACTTTCG CTTTCCCCCT CCCTATTGCC ACGGCGGAAC TCATCGCCGC CTGCCTTGCC 4921 CGCTGCTGGA CAGGGGCTCG GCTGTTGGGC ACTGACAATT CCGTGGTGTT GTCGGGGAAA 4981 TCATCGTCCT TTCCTTGGCT GCTCGCCTGT GTTGCCACCT GGATTCTGCG CGGGACGTCC 5041 TTCTGCTACG TCCCTTCGGC CCTCAATCCA GCGGACCTTC CTTCCCGCGG CCTGCTGCCG 5101 GCTCTGCGGC CTCTTCCGCG TCTTCGCCTT CGCCCTCAGA CGAGTCGGAT CTCCCTTTGG 5161 GCCGCCTCCC CGCATCGATA CCGTCGACCT CGATCGAGAC CTAGAAAAAC ATGGAGCAAT 5221 CACAAGTAGC AATACAGCAG CTACCAATGC TGATTGTGCC TGGCTAGAAG CACAAGAGGA 5281 GGAGGAGGTG GGTTTTCCAG TCACACCTCA GGTACCTTTA AGACCAATGA CTTACAAGGC 5341 AGCTGTAGAT CTTAGCCACT TTTTAAAAGA AAAGGGGGGA CTGGAAGGGC TAATTCACTC 5401 CCAACGAAGA CAAGATATCC TTGATCTGTG GATCTACCAC ACACAAGGCT ACTTCCCTGA 5461 TTGGCAGAAC TACACACCAG GGCCAGGGAT CAGATATCCA CTGACCTTTG GATGGTGCTA 5521 CAAGCTAGTA CCAGTTGAGC AAGAGAAGGT AGAAGAAGCC AATGAAGGAG AGAACACCCG 5581 CTTGTTACAC CCTGTGAGCC TGCATGGGAT GGATGACCCG GAGAGAGAAG TATTAGAGTG 51 GAGGTTTGAC AGCCGCCTAG CATTTCATCA CATGGCCCGA GAGCTGCATC CGGACTGTAC 5701 TGGGTCTCTC TGGTTAGACC AGATCTGAGC CTGGGAGCTC TCTGGCTAAC TAGGGAACCC

5761 ACTGCTTAAG CCTCAATAAA GCTTGCCTTG AGTGCTTCAA GTAGTGTGTG CCCGTCTGTT 5821 GTGTGACTCT GGTAACTAGA GATCCCTCAG ACCCTTTTAG TCAGTGTGGA AAATCTCTAG 5881 CAGCATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 5941 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 6001 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 6061 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 6121 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 6181 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 6241 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 6301 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 6361 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 21 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 81 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 6541 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 6601 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 6661 TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG 6721 TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT 6781 CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC 6841 GCGAGACCCA CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC 6901 CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG 6961 GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC 7021 AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG 7081 ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC 7141 TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT 7201 GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC 7261 AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT 7321 ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC 7381 TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC 7441 TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA 7501 AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT 7561 CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG 7621 ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG 7681 AAAAGTGCCA CCTGAC//

pSicoR PGK Puro其他相关慢病毒载体：

Tet-pLKO-neo pMD2.G

pLKO.1-TRC control

pCDH-MSCV-MCS-EF1-copGFP FUW-tetO-hOCT4 FUW

pLVX-AmCyan1-N1 pLVX-DsRed-Monomer-N1 pLVX-ZsGreen1-N1 pLVX-mCherry-C1

Tet-pLKO-puro pCMV-dR8.2-dvpr pLKO.1-hygro

pCDH-MSCV-MCS-EF1-copGFP-T2A-Puro FUW-tetO-hSOX2 pLVX-AcGFP1-N1 pLVX-DsRed-Express2-C1 pLVX-PAmCherry-C1 pLVX-IRES-ZsGreen1 pLVX-mCherry-N1

pPACKH1-GAG pLKO.1-GFP-shRNA pLKO.1-TRC FUW-tetO-hOKMS FUW-tetO-hKLF4 pLVX-AcGFP1-C1 pLVX-DsRed-Express2-N1 pLVX-PAmCherry-N1 pLVX-IRES-mCherry pLVX-tdTomato-C1

pLKO.1-puro pLVX-IRES-Puro

pLVX-EF1α-DsRed-Monomer-C1 pLVX-EF1α-mCherry-C1 pLVX-MetLuc Control pLVX-Het-2 pSIH1-H1-CopGFP pCDF1-MCS2-EF1-copGFP pRSV-rev

pLVX-DD-AmCyan1 Control pLVX-DD-tdTomato Control pLVX-TetOne-Puro-Luc pLVX-TetOne-Luc pLVX-rHom-1 pLVX-PTuner pLVX-Het-1

pCDH-CMV-MCS-EF1-RFP-T2A-Puro pCDH-MCS-T2A-Puro-MSCV pCDH-EF1-MCS-T2A-copGFP pLVX-TRE3G-mCherry pLenti6.3/V5-GW/EmGFP pLenti6.3-MCS-IRES2-EGFP VSV-G

pcDNA6.3-EmGFP-NC- II pLenti6.3-BveI miR pGIPZ FUGW pMDLg/pRRE

pLVX-TRE3G-Luc Control pSico pGensil-1 pPACKH1-REV

pLKO.1-puro-GFP-siRNA pCDH-MSCV-MCS-EF1-Puro FUW-tetO-hMYC FUW-M2rtTA pLVX-AmCyan1-C1 pLVX-DsRed-Monomer-C1 pLVX-ZsGreen1-C1 pLVX-IRES-tdTomato pLVX-tdTomato-N1 pLVX-Tight-Puro

pLVX-EF1α-DsRed-Monomer-N1 pLVX-EF1α-mCherry-N1

pLentilox 3.7 pLVX-IRES-Neo pLVX-EF1α-AcGFP1-N1 pLVX-EF1α-IRES-mCherry pLVX-MetLuc

pLVX-DD-AcGFP1-Actin pCDH-EF1-MCS-T2A-Puro pLOX-CWBmi1 pMDLg-pRRE

pLVX-DD-AmCyan1 Reporter pLVX-PTuner-Green pLVX-TetOne pLVX-rHom-Nuc1 pLVX-Hom-Nuc1 pLVX-PTuner2

pLVX-CherryPicker Control pCDH-CMV-MCS-EF1-Hygro pCDH1-MCS2-EF1-copGFP pWPXL

pLenti6.3-EmGFP-BveI miR pLenti6.3-MCS pLVX-shRNA2 pSico PGK Puro pcDNA6.2-EmGFP-NC- I pLenti6.3-MCS-IRES2-DsRed2 pLP2 pFUGW pLentG-KOSM pLVX-TRE3G-IRES pSicoR

pLVX-EF1α-IRES-Puro pLVX-Het-Mem1 pPRIME-TREX-GFP-FF3 pCDH-CMV-MCS-EF1-copGFP pLOX-TERT-iresTK pCDH-EF1-MCS-(PGK-Puro) pLVX-Hom-1 pLVX-mCherry-Actin pLVX-CherryPicker1

pCDH-CMV-MCS-EF1-copGFP-T2A-Puro pLVX-PTuner2-C

pLVX-DD-ZsGreen1 Control pCDH-UbC-MCS-EF1-Hygro pCDH-CMV-MCS-EF1-RFP

pLVX-Tet-On-Advanced pLVX-IRES-Hyg pLVX-EF1α-AcGFP1-C1 pLVX-EF1α-IRES-ZsGreen1 pLVX-Hom-Mem1 pPRIME-TET-GFP-FF3 pCDH-CMV-MCS-EF1-Puro pLOX-CW-CRE pLL3.7

pLVX-DD-tdTomato Reporter pLVX-CherryPicker2 pLVX-TetOne-Puro pLVX-rHom-Sec1 pLVX-Het-Nuc1

pLVX-DD-ZsGreen1 Reporter pLVX-Tet3G

pCDH-CMV-MCS-EF1-Neo pCDF1-MCS2-EF1-Puro pLVX-TRE3G-ZsGreen1 pLenti6/V5-GW/lacZ pLenti6.3-DsRed2-BveI miR psPAX2

pcDNA6.2-DsRed2-MCS1 miR pcDNA6.2-EmGFP-BsaI miR pLEX-MCS pLP1

pLOX-Ttag-iresTK pCMV-dR8.91 pCgpv pLVTHM

pCDF1-MCS2-EF1-copGFP pLVX-shRNA1

pcDNA6.2-DsRed2-BsmBI miR pLVX-TRE3G pLP/VSVG

pcDNA6.2-EmGFP-MCS1 miR pcDNA6.2-BsaI miR pTRIPZ LeGO-iC2 pLKO.3G pLVX-Puro pSicoR PGK Puro pLVTH